Methods: Five kinds of conventional extracting solution of Hirudo ( alcohol and water extracting, alcohol refluxing, water extracting, water extracting-alcohol precipitating, percolation filtration) were used in this study. 方法:以五种常用中药提取法(水醇双提法、乙醇回流法、水提法、水提醇沉法、渗滤法)提取水蛭;
Conclusions: Five kinds of conventional extracting solution of Hirudo all can inhibit the proliferation of LEC cultivated in vitro, and inhibition of water extracting-alcohol precipitating solution and percolation filtration solution was more strong among them. 结论:五种水蛭不同方法提取液均能不同程度抑制体外培养LEC的生长,其中水提醇沉液、渗滤液作用更为明显。